In the past two decades, immunological analysis methods have experienced remarkable progress, particularly with the development of techniques that use labeled antigens and antibodies. These advancements have significantly improved the sensitivity and specificity of many traditional analytical methods, making them less competitive in modern diagnostics.
Following the introduction of immunofluorescence (IFA) in the 1950s and radioimmunoassay (RIA) in the 1960s, enzyme-labeled techniques for antigens or antibodies were developed in the early 1970s. The high catalytic efficiency of enzymes allowed a single enzyme molecule to catalyze hundreds of substrate molecules within minutes, leading to signal amplification. This made it possible to detect even minute amounts of antigen or antibody quickly. This technique became known as Enzyme-Linked Immunosorbent Assay (ELISA), which was widely adopted and reported by researchers such as Van Weemen, Schuurs, Engvall, and Perlman from the early 1970s onwards.
ELISA has since become a cornerstone in immunodiagnosis, with successful applications in detecting infectious diseases, parasitic infections, and non-communicable conditions caused by various pathogens. It is also used for quantifying both large and small molecular antigens. Due to its high sensitivity, specificity, simplicity, speed, stability, and ease of automation, ELISA is not only ideal for clinical specimen testing but also suitable for large-scale sero-epidemiological studies, where hundreds or even thousands of samples can be processed in a single day. Moreover, ELISA is useful for detecting both antibodies and circulating antigens in body fluids, making it a valuable tool for early diagnosis.
The application of ELISA spans four main areas:
1. Immune enzyme staining for localizing intracellular components.
2. Studying the synthesis of anti-enzyme antibodies.
3. Observing small-scale immunoprecipitation.
4. Quantitative detection of antigens or antibodies in body fluids.
Shanghai Tiancheng Technology Co., Ltd. has built a strong reputation in the national biological and medical community through its brand image, excellent pricing, and reliable service. The company now offers a comprehensive range of diagnostic reagents, supported by a well-stocked warehouse, professional inspectors, quality administrators, and experienced sales staff. With a wide selection of ELISA products, Tiancheng ensures top-quality solutions tailored to different research needs.
**Why choose Tiancheng?**
**1. Wide Range of Products**
Tiancheng provides ELISA kits for human and animal testing, including pet diagnostic reagents, avian disease detection kits, tuberculin, canine disease kits, IDS animal diagnostic kits, and food safety testing series. After years of development, the company has established partnerships with numerous global and domestic manufacturers, such as R&D, Gaoz, eBioscience, BioSource, DSL, BioCheck, ADL, CayMan, Biovision, Biovendor, RB, MI, ESBL, Phoenix, Enzo, GBD, PPI, DRG, RayBiotech, Millipore, BiOwen, Diaclone, IDS, PeProTech, IBL, Weirun Run, BenDer, MabTech, IDEXX, Synbiotics, and more.
**2. Competitive Pricing**
Whether you need low-cost boxes for preliminary experiments or premium kits for publication and product development, Tiancheng offers options to suit every budget. Delivery times vary depending on the brand, typically ranging from 7 to 10 days.
**3. Comprehensive Equipment Support**
For those starting ELISA tests or replacing equipment, Tiancheng offers a full range of support, including microplate readers, washers, refrigerators, incubators, and other lab essentials. For example, the Finland Multiskan MK3 Leibo microplate reader, BIO-RAD 680 Böhler reader, and BIO-TEK ELX800 reader are available. Matching washing machines and storage equipment are also provided.
**4. Customized Services**
Tiancheng welcomes collaboration with clinical and research institutions at all levels. The company prides itself on offering fast turnaround times—within three working days of receiving specimens, customers receive their results. When sending samples, it’s important to provide details such as sample number, processing method, test items, whether duplicates are needed, contact information, and whether samples will be returned after testing.
**Specimen Collection Guidelines**
Before collecting samples, ensure the target compounds are stable. If testing on the same day, store samples at 4°C. For long-term storage, keep them at -20°C or -70°C, avoiding repeated freezing and thawing. Most samples can be stored for up to 48 hours at 2–8°C, one month at -20°C, and six months at -70°C. Some hormone samples may require the addition of aprotinin.
**Liquid Specimens**
Include serum, plasma, urine, pleural fluid, ascites, cerebrospinal fluid, and cell culture supernatants. Each sample volume should be approximately 100 µl per test. Proper preparation includes centrifugation to remove sediments.
**Serum Preparation**
Allow whole blood to clot at room temperature for 10–20 minutes, then centrifuge at 2000–3000 rpm for 20 minutes. Collect the supernatant carefully.
**Plasma Preparation**
Use anticoagulants like EDTA, sodium citrate, or heparin. Add 10% anticoagulant (0.1M sodium citrate, 1% heparin, or 2.0% EDTA.Na2), mix, and centrifuge for 20 minutes.
**Urine, Cerebrospinal Fluid, and Ascites**
Collect in sterile tubes and centrifuge to remove sediment before analysis.
**Cell Culture Supernatant**
Collect in sterile tubes, centrifuge, and process as needed. For intracellular components, lyse cells via freeze-thaw cycles before centrifugation.
**Tissue Specimens**
Homogenize tissues in PBS with protease inhibitors, centrifuge, and collect the supernatant.
With its extensive product range, competitive pricing, and expert support, Shanghai Tiancheng Technology Co., Ltd. is your trusted partner in ELISA testing. Whether you're conducting basic research or clinical diagnostics, we offer a complete solution tailored to your needs.
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