**Factor VIII-Related Antigen (FVIII-Ag) ELISA Kit Instructions**
**Purpose:**
This ELISA kit is designed for the quantitative determination of Factor VIII-Related Antigen (FVIII-Ag) in human serum, plasma, and other biological fluids. It provides a reliable and sensitive method to assess FVIII-Ag levels, which are critical in diagnosing and monitoring coagulation disorders such as hemophilia A.
**Experimental Principle:**
The kit utilizes a double-antibody sandwich ELISA technique. A microtiter plate is pre-coated with a specific antibody against FVIII-Ag. The sample is added, allowing FVIII-Ag to bind to the immobilized antibody. A horseradish peroxidase (HRP)-labeled secondary antibody then binds to the captured antigen, forming an immune complex. After washing away unbound components, a TMB substrate is added. Under the catalytic action of HRP, TMB turns blue and then changes to yellow upon acidification. The intensity of the color is directly proportional to the concentration of FVIII-Ag in the sample. The absorbance is measured at 450 nm using a microplate reader, and the concentration is calculated based on a standard curve.
**Application Notes:**
This kit is suitable for clinical laboratories and research settings where accurate quantification of FVIII-Ag is required. It ensures high specificity and reproducibility, making it ideal for both diagnostic and scientific applications.
**Additional Information:**
This ELISA kit is part of a broader range of immunoassay products, including kits for Leptin Receptor (LR/Ob-R), Anti-Myocardial Antibody (AMA), Pertussis Toxin (PT), Superoxide Dismutase (SOD), Anti-Deoxyribonucleoprotein Antibody (DNP-Ab), β-Mannosidase (β-Manase), Free β-HCG, Serum Amyloid P (SAP), Anti-Phosphatidylserine Antibody (APSA), Ceruloplasmin (CP/CER), Tuberculosis Antibody IgG (TB-Ab IgG), and Anti-Insulin Receptor Antibody (AIRA). Each kit follows similar principles but is tailored for specific biomarkers, ensuring accurate and consistent results across various applications.
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