Rabbit White (GFAP) ELISA Test Company is a professional supplier of enzyme-linked immunosorbent assay (ELISA) kits and related reagents. Our products are designed with high efficiency, sensitivity, specificity, and excellent repeatability, ensuring reliable results across a wide range of sample types, including body fluids, tissue homogenates, cell culture supernatants, urine, and more. All our customers can benefit from competitive pricing and special offers on experimental kits, making our ELISA solutions both cost-effective and scientifically valuable.
This ELISA kit is specifically intended for the quantitative detection of human anti-neutrophil cytoplasmic antibody (cANCA) in various biological samples such as cell culture media, body fluids, tissues, serum, and plasma. It is exclusively for research use and not for diagnostic purposes.
**Preparation of Experimental Materials and Reagents:**
1. **Instruments and Materials:**
- Microplate reader (preheated for 30 minutes before use)
- Microtiter plates
- Pipettes and pipette tips
- Distilled or deionized water
- Filter paper
2. **Reagent Preparation:**
- Dilute the working solution at a ratio of 1:30 before use. Ensure proper mixing and storage according to the manufacturer's instructions.
**Sample Collection, Processing, and Storage:**
1. **Cell Culture Supernatant:**
Collect the supernatant using sterile tubes, centrifuge at 1000 × g for 15 minutes, and carefully transfer the supernatant for testing.
2. **Cells:**
Wash cells three times with PBS, adjust the concentration to approximately 10â´â€“10ⶠcells/mL. After repeated freeze-thaw cycles or ultrasonic disruption, centrifuge the lysate to collect the supernatant.
3. **Serum:**
Allow blood to clot at room temperature, then centrifuge at 1000 × g for 15 minutes to obtain the supernatant.
4. **Plasma:**
Use EDTA or sodium citrate as anticoagulant. Mix thoroughly for 10–20 minutes, then centrifuge at 1000 × g for 15 minutes to collect the plasma.
5. **Body Fluids:**
Include pleural fluid, ascites, cerebrospinal fluid, and other secretions. Centrifuge in endotoxin-free tubes at 1000 × g for 15 minutes and collect the supernatant.
6. **Tissue Homogenate:**
Grind 1 g of tissue in 9 mL of PBS (0.1 M, pH 7.4), mix thoroughly, and centrifuge to obtain the supernatant. Avoid using NaN₃, as it may inhibit horseradish peroxidase (HRP) activity.
**Storage Instructions:**
If immediate testing is not possible, aliquot the samples and store them at -70°C to prevent multiple freeze-thaw cycles. If precipitation occurs, re-centrifuge before use. Avoid using hemolyzed or lipemic samples. If the sample contains particulates, filter or centrifuge prior to testing. Thaw samples at room temperature only; avoid heating above 37°C. Ensure complete and even thawing before proceeding with the assay.
reception desk, entrance desk ,welcome station console table, greeting table, entrance organizer
Foshan Beausimpe Furniture Co., Ltd , https://www.minottiitaly.com