Principle and operation of low temperature incubator

Low temperature incubator refrigeration working principle:

The refrigeration system in a low-temperature incubator operates on the basis of the reverse Carnot cycle, which consists of two isothermal and two adiabatic processes. The refrigerant is first compressed by the compressor under adiabatic conditions, increasing its pressure and temperature. This high-pressure refrigerant then flows through a condenser, where it releases heat to the surrounding environment in an isothermal process. After passing through an expansion valve, the refrigerant undergoes adiabatic expansion, causing its temperature to drop significantly. Finally, the cold refrigerant absorbs heat from the object inside the incubator via the evaporator, lowering its temperature. This cycle repeats continuously, maintaining the desired low-temperature environment. The test chamber employs a dual-stage Freon refrigeration system using a French-made Taikang fully sealed compressor. Energy adjustment technology is integrated into the design to ensure stable operation, optimize energy consumption, and maintain consistent cooling performance. A balanced temperature control system (BTHC) is used, allowing the control system to adjust heater output based on PID calculations, ensuring dynamic balance between heating and cooling according to the set temperature.

Low temperature incubator operation process:

The control panel includes several key functions: 1. **Power switch**: Labeled "POWER," this switch should be turned to the "I" position to activate the device. 2. **Temperature setting**: Use the "SET TEMPERATURE" button with UP/DOWN arrows to set and adjust the desired temperature. 3. **Over-temperature protection**: The "SET OVERTEMPERATURE" function allows adjustment from 0 to 10, providing a secondary safety layer against overheating. 4. **Heating lamp**: Indicates when the heating function is active. 5. **Over-temperature protection lamp**: Lights up when the over-temperature protection mechanism is engaged. 6. **Day/Night Program Controller**: Divides the 24-hour cycle into two 12-hour periods for day and night settings. 7. **Lighting controller**: Manages lighting or darkness mode over the full 24-hour period. 8. **Fuse**: Located near the power input at the bottom, it protects against voltage fluctuations and must be replaced if blown.

Basic operating procedures for cryogenic incubators:

1. Turn on the power and set the over-temperature protection knob to the maximum position. 2. Place a calibrated thermometer in the center of the incubator, avoiding contact with shelves or walls. 3. Ensure proper air circulation around the incubator to achieve uniform temperature distribution. 4. Avoid placing open containers inside, as moisture can lead to frost buildup, affecting temperature stability. 5. Set the desired temperature by entering the temperature interface and adjusting using the UP/DOWN buttons. If no action is taken within five seconds, the display will return to the actual temperature. Allow at least 24 hours for stabilization. 6. Perform calibration after stabilization. Compare the displayed temperature with the thermometer reading and adjust if necessary by entering calibration mode. 7. Adjust the over-temperature protection by turning the knob clockwise to the maximum, then counterclockwise until the protection light turns on. Then turn it back slightly to ensure it activates about 1°C above the set point. 8. Time settings for day/night and lighting are controlled independently, each divided into 96 segments representing 15 minutes. Adjust the dials accordingly to match the desired schedule. 9. Connect external equipment with a maximum current of 1A. Be cautious of additional heat generated by devices that may affect the incubator's performance. 10. Under normal operation, the outer wall of the incubator may feel warmer than the ambient temperature. This detailed setup ensures precise temperature control, safe operation, and reliable performance in low-temperature environments.

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