Shanghai Chuangsai Technology: The method of using dialysis bags

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Product packaging and price

Item number

product name

specification

Catalog price (yuan)

Price (yuan)

S1666

MD34 dialysis bag (width: 34mm, MW: 14000)

120

C55-MD34-7

MD34 dialysis bag (width: 34mm, MW: 7000)

210

192

C55-MD34-3.5

MD34 dialysis bag (width: 34mm, MW: 3500)

240

168

Dialysis bag use method 1

The dialysis is to separate the macromolecular proteins with a semi-permeable membrane (dialysis bag). Dialysis is the easiest method to remove salts, small amounts of organic solvents, small biomolecular impurities, and concentrated samples during biomacromolecule preparation. Pre-treatment: To prevent cracking, it is treated with 10% glycerin at the factory, and contains a very small amount of sulfides, heavy metals and some impurities with UV absorption. They are harmful to proteins and other biologically active substances and must be removed before use. It can be boiled for 1 hour with 50% ethanol, then washed with 50% ethanol, 0.01 mol/L sodium bicarbonate and 0.001 mol/L EDTA solution, and finally rinsed with distilled water. Experiments have shown that 50% ethanol treatment is particularly effective in removing impurities with UV absorption. After washing, the dialysis bag can be stored in distilled water at 4 Â° C. If it is not used for a long time, a small amount of NaN2 can be added to prevent growth. The washed and dried dialysis bag is easy to crack when bent, and must be carefully inspected when used. It can be reused without leaking.

If the new dialysis bag is not specially treated as above, it can be boiled in boiling water for five to ten minutes, then washed with distilled water and used. When using, one end is fastened with rubber band or wire rope. It can also be clamped with a special dialysis bag clamp. Fill the water from the other end, press it slightly with your fingers, check it without leaking, and then put it into the solution to be dialysate. Usually To leave a space of one-third to one-half, in case of dialysis, when the small molecular weight of dialysis is large, the water and buffer outside the bag excessively enter the bag to break the bag. When the protein solution containing a high salt content is dialyzed overnight, a 50% increase in volume is normal. In order to speed up the dialysis, in addition to multiple changes to the dialysate, magnetron agitation can also be used. The dialysis container is larger, and large beakers, large tubes, and plastic buckets can be used. For the dialysis of a small volume of solution, a glass rod of two burning gardens or a glass tube sealed at both ends may be placed in the bag to make the dialysis bag sink below the liquid surface.

Dialysis bag use method 2

1. Cut the dialysis tube into small pieces of appropriate length (10-20 cm) to form a dialysis bag.

2. Boiling the dialysis bag for 10 min in a large volume of 2% (m/v) sodium bicarbonate and 1 mmol/LEDTA (pH = 8).

3. Rinse the dialysis bag thoroughly with distilled water.

4. Boil the dialysis bag in 1 mmol/LEDTA (pH=8) for 10 min.

5. The dialysis bag is stored at 4 degrees after cooling and should ensure that the dialysis bag is always submerged in the liquid.

Note: Always wear gloves when using the dialysis bag from this step.

6. Wash the inside and outside of the dialysis bag with distilled water before use.

Note: It is also possible to place the dialysis bag in a jar filled with water, loosen the cap, and autoclave at 20 psi (1.40 kg/cm2) for 10 min instead of the fourth step with 1 mmol/LEDTA (pH=8) for 10 min. operating.

After washing, the dialysis bag can be stored in distilled water at 4 Â° C. If it is not used for a long time, a small amount of NaN2 can be added to prevent growth. The washed and dried dialysis bag is easy to crack when bent, and must be carefully inspected when used. It can be reused without leaking.

If the new dialysis bag is not specially treated as above, it can be boiled in boiling water for five to ten minutes, then washed with distilled water and used.

The dialysis effect was examined by inspecting (NH4)2SO4 with 1% BaCl2 and checking for NaCl, KCl, etc. with 1% AgNO3.

Protein concentration technology commonly used in immunology

Dialysis bag concentration method

The use of dialysis bags to concentrate protein solutions is the most widely used. Place the protein solution to be concentrated in a dialysis bag (no dialysis bag can be replaced with cellophane), ligation, and polymer (6 000-12 000) polymers such as polyethylene glycol (carbon wax), polyvinylpyrrole, alkanone, etc. Sucrose can be sprinkled outside the dialysis bag. The water absorbing agent may also be formulated into a solution having a concentration of 30% to 40%, and the dialysis bag containing the protein solution may be placed. After the water absorbing agent is used, it can be placed in a thermostat for drying or natural drying and can be reused.

Ultrafiltration membrane concentration method

This method utilizes a microporous cellulose membrane to filter out water by high pressure, and the protein remains on the membrane for concentration purposes. There are two ways to concentrate: one is to insert the cellulose acetate membrane into the high pressure filter and filter under constant stirring; the other is to put the protein solution into the dialysis bag and built in the vent of the vacuum dryer, negative The air is pumped to make the liquid in the bag ooze out.