The research group of Professor Wan Jianmin of Nanjing Agricultural University has made important progress in the research of rice gluten sorting mechanism. Recently, the internationally renowned botanical authoritative journal "The Plant Cell" published online research results entitled "GLUTELIN PRECURSOR ACCUMULATION3 Encodes a Regulator of Post-Golgi Vesicular Traffic Essential for Vacuolar Protein Sorting in Rice Endosperm". Dr. Ren Yulong, Associate Professor Wang Yihua and Dr. Liu Feng were the first authors of the paper, and Professor Wan Jianmin was the corresponding author.
Rice contains a lot of storage protein, which is the second largest substance in rice after starch. Among them, gluten is the storage protein with the highest content in rice seeds, accounting for more than 70% of the total protein in the seeds. It plays an important role in the quality of rice and its nutritional value. Glutin is first synthesized in the form of a 57kDa precursor in the endoplasmic reticulum of endosperm cells. After a complex vesicle transport process, it is transported into the protein storage vacuole (PSV). . However, we know very little about the molecular mechanism of gluten precursor vesicle transport and directed sorting.
Researchers from the Jianmin Wan group obtained a series of mutants with abnormal accumulation of rice glutenin precursors through extensive screening, one of which was named glutelin precursor accumulation3 (gpa3). Cytological studies have found that in this mutant, the dense vesicles (DV, dense vesicles) derived from Golgi carrying gluten precursors were erroneously sorted near the plasma membrane of the cell, and gluten and other molecules were sorted into The extracellular space has formed an abnormal structure of paramural body (PMB, paramural body), indicating that gpa3 is a mutant of rice gluten and post-Golgi sorting defect; map-based cloning reveals that the GPA3 gene encodes a plant-specific Kelch- The repeat motif protein is named OsKelch1; subsequent functional studies have found that GPA3 / Kelch1 protein can form a functional protein complex with the small G protein GPA1 / Rab5a and its upstream activator GPA2 / VPS9a reported by our research group. Regulate the directional transport of DV to PSV.
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