"Twelfth Five-Year Plan" 863 project made important progress in the field of biological and pharmaceutical technology

The "12th Five-Year" 863 project biological resources and safety theme project "Key Technology for the Development and Utilization of Special Biological Resources" aims at the development of key products and processes, focusing on special fungal resources, high-value economic plants, special medicinal resources and special microbial resources Conduct research to establish a batch of special biological resource functional strain libraries, functional gene libraries and molecular enzyme libraries with Chinese characteristics and independent intellectual property rights, form a batch of technical platforms for the development of special biological resources, and develop a batch of special biological resources Value-added products and large-scale demonstration production lines. A year after the project was launched, a series of important results have been achieved.

In the establishment of functional strain library, gene library and molecular enzyme library: Agrobacterium-mediated Ganoderma lucidum transformation system was established by genetic engineering, so that the content of ganoderic acid in the engineering strain was increased by 2 times compared with the wild type strain; Cordyceps sinensis was obtained The whole genome sequence of Trichosporium chinense and the transcriptome sequence under different culture conditions have determined the anabolism pathways of Trichosporium mannitol, Cordycepin, purine nucleosides, pyrimidine nucleosides, and unsaturated fatty acids 72 functional genes; constructing genetically modified strains of engineering bacteria producing doramectin, which increased the industrial fermentation potency of doramectin from 1200mg / L to 1300mg / L; establishing 8 snails of marine snails 56 new conotoxin functional genes were found in the toxic tube cDNA gene library and 7 conotoxin natural active peptide libraries.

In terms of new processes and technologies for the development and utilization of special biological resources: through the pretreatment strategy of acidolysis-acetylation-acidolysis, a new technology for the separation and purification of ganoderma acid was established, so that the quality and content of ganoderma acid T in the crude extract The resolution is increased by 4 times and 7 times respectively; the extraction and purification process of Cordycepin in Cordyceps militaris has been optimized, and the recovery rate of Cordycepin has reached more than 90%. Technical level; established a rapid detection method of microbial intracellular oil, microbial oil full component analysis platform and oil-producing microbial intracellular small molecule substance analysis platform.

In terms of industrialization and demonstration: established a production line of Cordyceps sinensis Chinese trichosporium powder, with the production and sales capacity of Cordyceps sinensis trichosporium Chinese powder; established a 100 square meters Cistanche seed production line, net bag container seedlings The seedling rate is as high as 90% or more; a small-scale continuous system using organic waste water and fermentation by-product CO2 to cultivate economic microalgae is built, and on this basis, an energy microalgae using pig biogas slurry as a medium is constructed Pilot breeding project; led the formation of "China Cordycepin Industry-University-Research Cooperation Strategic Alliance", which laid the foundation for the research and industrialization of Cordycepin.

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