Method 1 Double antibody sandwich method 1. Coating: Dilute the antibody to protein content of 1-10 μg / ml with 0.05MPH9. Carbonate coating buffer. Add 0.1ml to the reaction well of each polystyrene plate, overnight at 4 ℃. The next day, the solution in the well was discarded and washed 3 times with washing buffer for 3 minutes each time. (Referred to as washing, the same below). 2. Add sample: add 0.1ml of the diluted test sample to the above-mentioned coated wells and incubate at 37 ℃ for 1 hour. Then wash. (Make blank wells, negative control wells and positive control wells at the same time). 3. Add enzyme-labeled antibody: add 0.1ml of freshly diluted enzyme-labeled antibody (dilution after titration) to each reaction well. Incubate at 37 ° C for 0.5 to 1 hour and wash. 4. Add substrate to develop color: add 0.1ml of temporarily prepared TMB substrate solution to each reaction well at 37 ℃ for 10 to 30 minutes. 5. Terminate the reaction: Add 0.05ml of 2M sulfuric acid to each reaction well. 6. Judgment of results: The results can be directly observed with the naked eye on a white background: the darker the color of the reaction well, the stronger the positive degree, and the negative reaction is colorless or extremely light. According to the depth of the color, "+", "-" Indicates. OD value can also be measured: on the ELISA detector, at 450nm (410nm if ABTS color is developed), the OD value of each well is measured after zero adjustment with a blank control well, if it is greater than 2.1 times the specified negative control , That is positive. Method two: Indirect method 1. Dilute the known antigen to 1 ～ 10μg / ml with coating buffer, add 0.1ml to each well, overnight at 4 ℃; 2. Wash 3 times the next day; 3. Add a certain dilution of the sample to be tested (Unknown antibody) 0.1ml in the above-coated reaction wells, incubate at 37 ° C for 1 hour, wash; 4. (Also do blank, negative and positive well control) in the reaction wells, add freshly diluted enzyme label 0.1ml of secondary antibody (anti-antibody); incubate at 5.37 ° C for 30-60 minutes and wash; 6. 'Last wash with DDW. Interleukin 6Elisa kit related products: human interleukin 12 (IL-12 / P70) ELISA kit human interleukin 12 (IL-12 / P40) ELISA kit human interleukin 11 (IL-11) ELISA kit human interleukin 10 (IL- 10) ELISA kit chicken interleukin 6 (IL-6) ELISA kit chicken interleukin 4 (IL-4) ELISA kit chicken interleukin 2 (IL-2) ELISA kit rat interleukin 6 (IL-6) ELISA kit Rat Interleukin 4 (IL-4) ELISA Kit Rat Interleukin 2 (IL-2) ELISA Kit Rat Interleukin 1α (IL-1α) ELISA Kit Rat Interleukin 18 (IL-18) ELISA Kit Rat Interleukin 10 (IL-10) ELISA Kit Mouse Interleukin 6 (IL-6) ELISA Kit Mouse Interleukin-5 (IL-5) ELISA Kit Mouse Interleukin 4 (IL-4) ELISA Kit Mouse Interleukin 3 (IL-3) ELISA kit mouse interleukin 2 (IL-2) ELISA kit mouse interleukin 1α (IL-1α) ELISA kit order method: 1. After confirming the product, you can directly contact us online, or Contact by phone to confirm the product you purchased.
Dining Room Sets,Wooden Table And Chairs,Dining Room Chairs,6 Piece Dining Set
Foshan City Misirui Furniture Co., Limited , https://www.misiruifurniture.com