Many organic compounds have characteristic absorption spectra in the ultraviolet region, so ultraviolet spectrophotometry can be used for qualitative identification, structural analysis and quantitative determination of organic substances. The basis of quantitative determination by ultraviolet spectrophotometry is Beer's law. First determine the ultraviolet absorption spectrum of the compound and determine the maximum absorption wavelength. At the selected wavelength, the working curve of the compound solution is made, and the content of the compound in the test solution is determined according to the absorbance value of the test solution measured under the same conditions.
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