The detection principle kit uses double antibody one-step sandwich enzyme-linked immunosorbent assay (ELISA). To the coated microwells precoated with T-TAU capture antigen, the specimen, standard, and HRP-labeled detection antibody were added in sequence, after incubation and thorough washing. The color is developed with the substrate TMB, which is converted into blue under the catalysis of peroxidase and into the final yellow under the action of acid. The shade of the color is positively correlated with the porcine total TAU protein (T-TAU) in the sample. Measure the absorbance (OD value) at 450nm with a microplate reader to calculate the sample concentration.
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