One-step centrifugal paraffin scavenger products and features One of the challenges of using paraffin-embedded tissue as a material for PCR is how to effectively remove paraffin, because the residual paraffin will not only affect DNA extraction, but also interfere with PCR amplification. Conventional dewaxing methods generally require 1-2 hours and require multiple centrifugations, each of which will cause the loss of samples. This product requires only one step of centrifugation, which overcomes the shortcomings of conventional methods. The characteristics are summarized as follows: 1. Fast and simple, only need to be centrifuged once, eliminating repeated fluid exchange and centrifugation, reducing the loss of samples. 2. The dewaxing effect is good, which is equivalent to the classic xylene / ethanol method. 3. Ready to use, customers do not need to prepare additional reagents. Specifications and ingredients Ingredients 100 times packaging solution A 100 mL solution B 7.5 mL user manual 1 shipment and storage Transportation and storage at room temperature, valid for one year. Bring your own reagents No use method 1. Add 1 mL of solution A to a 1.5 mL plastic centrifuge tube. 2. Put a piece of paraffin-embedded tissue section no more than 25 um. 3. Shake for 10 seconds at maximum speed. 4. Add 75 uL of solution B. 5. Shake for 10 seconds to mix. 6. Centrifuge at 7000 g for 2 minutes, carefully discard the supernatant. 7. The vacuum dryer dries up the residual liquid in the centrifuge tube. This step takes about an hour. 8. The obtained paraffin-embedded tissue sections can be used for DNA extraction.
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