At present, the more commonly used method in ELISA experiments is to detect by means of ELISA kits and related experimental instruments, which improves the accuracy of the experiment to a certain extent, and is more economical and practical. Protein, the equipment needed in the experiment is not cheap, nor is it affordable for everyone. Nature magazine recommended a highlight study. Molly Stevens and Roberto de la Rica of the Imperial College London invented a new ELISA technology that can read the test results only with the naked eye. Their research was published in the Nature sub-Journal Nature Nanotechnology on. In their method, the enzyme in the ELISA is used to control the accumulation of gold nanoparticles, which will appear blue if the target protein is present, and red if the target protein is not present. Observation with the naked eye certainly cannot be quantified, but their technology can detect HIV proteins at concentrations as low as 1 ag (attogram) / ml, which is quite sufficient for clinical testing.
The researchers used this technique to detect ultra-low concentrations (1 × 10-18 g / ml) of prostate-specific antigen PSA and HIV-1 capsid antigen p24 in whole blood. And in the case of nucleic acid detection, which cannot be detected by the gold label method, the technology can detect the HIV-1 capsid antigen p24 in the blood of HIV patients with low viral load, and can make a judgment directly with the naked eye.
This technology has brought the gospel to laboratories with limited funds. It can quickly detect disease marker proteins in clinical samples at a low cost, without the need for more expensive and complicated instruments.
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