ELISA kit use:
The human HP-IgG ELISA kit is used for in vitro quantitative detection of HP-IgG in cell supernatants in serum, plasma, tissues, buffers, and various body fluids. This kit can detect natural and recombinant HP-IgG. This kit is designed for scientific research, not for clinical diagnosis.
Principle of ELISA kit:
The human HP-IgG kit is a solid-phase sandwich enzyme-linked immunosorbent assay (ELISA). Standards with known HP-IgG concentrations and samples with unknown concentrations are added to microwell enzyme plates for detection. Incubate HP-IgG and biotin-labeled antibodies first. After washing, HRP labeled with avidin was added. After incubation and washing, the unbound enzyme conjugate is removed, and then substrates A and B are added to act simultaneously with the enzyme conjugate. Produce colors. The color depth is proportional to the concentration of HP-IgG in the sample.
ELISA kit contents and preparation
Kit components (stored at 2-8 ° C)
96-well configuration
48 hole configuration
Preparation
96/48 serving plate
1 board (96T)
Half plate (48T)
Ready-to-use
Plastic membrane cover
1 piece
Half block
Ready-to-use
Standard product: 8.0IU / L
1 bottle (0.6ml)
1 bottle (0.3ml)
Follow the instructions for sparse
Blank control
1 bottle (1.0ml)
1 bottle (0.5ml)
Ready-to-use
Standard dilution buffer
1 bottle (5ml)
1 bottle (2.5ml)
Ready-to-use
Biotin-labeled anti-HP-IgG antibody
1 bottle (6ml)
1 bottle (3.0ml)
Ready-to-use
Streptavidin-HRP
1 bottle (10ml)
1 bottle (5.0ml)
Ready-to-use
Wash buffer
1 bottle (60ml)
1 bottle (30ml)
Dilute according to the instructions
Substrate A
1 bottle (6.0ml)
1 bottle (3.0ml)
Ready-to-use
Substrate B
1 bottle (6.0ml)
1 bottle (3.0ml)
Ready-to-use
Stop solution
1 bottle (6.0ml)
1 bottle (3.0ml)
Ready-to-use
Bring your own materials
1. Distilled water.
2. Sampler: 5ul, 10ul, 50ul, 100ul, 200, 500u, 1000lul.
3. Oscillator and magnetic stirrer etc.
Custom Aluminum Frame,Aluminum Frame,Cheap Aluminum Frame,Extrusion Aluminum Frame
shaoxing gesheng wire mesh co., ltd , https://www.gscreenmesh.com