Experimental principle of human glutamate dehydrogenase (GDH / GLDH) ELISA kit
English name: Human Glutamate dehydrogenase; GDH / GLDH ELISA
1. The level of human glutamate dehydrogenase (GDH / GLDH) ELISAKit in the specimens was measured by double antibody sandwich method.
2. First coat the microplate with purified human glutamate dehydrogenase (GDH / GLDH) ELISAKit antibody to make a solid phase antibody.
3. Then add glutamate dehydrogenase (GDH / GLDH) ELISAKit to the microwells coated with mAb in turn, and then combine with HRP-labeled glutamate dehydrogenase (GDH / GLDH) ELISAKit antibody to form antibody-antigen -Enzyme-labeled antibody complex, after thorough washing, add substrate TMB to develop color. TMB is converted into blue under the catalysis of HRP enzyme, and into the final yellow under the action of acid.
4. The color depth is positively correlated with the glutamate dehydrogenase (GDH / GLDH) ELISAKit in the sample.
5. Finally, the absorbance (OD value) is measured with a microplate reader at a wavelength of 450 nm, and the concentration of glutamic acid dehydrogenase (GDH / GLDH) ELISAKit in the sample is calculated from the standard curve
.
Objective: To determine the level of glutamate dehydrogenase (GDH / GLDH) ELISAKit in human serum, plasma and related liquid samples.
6. Serum: Blood coagulates naturally at room temperature for 10-20 minutes, centrifuged for about 20 minutes (2000-3000 rpm). Collect the supernatant carefully and centrifuge again if a precipitate appears during storage. Avoid any cell stimulation during the operation. Use test tubes free of pyrogens and endotoxins.
7. Plasma: EDTA or sodium citrate should be selected as the anticoagulant according to the requirements of the specimen. After mixing for 10-20 minutes, centrifuge for about 20 minutes (2000-3000 rpm) to remove particles. Collect the supernatant carefully, and centrifuge again if there is any precipitate.
safety:
1. Avoid direct contact with stop solution and substrate. If you accidentally come into contact with these liquids, please rinse them with water as soon as possible.
2. Do not eat, drink, smoke or use cosmetics during the experiment.
3. Do not touch any ingredients in the kit with your mouth.
4 Keep Elisa kit away from children
Bring your own items:
1. 37 ℃ incubator
2. Standard specification microplate reader
3. Precision pipette and disposable tip
4. Distilled water
5. Disposable test tube
6. Absorbent paper
Storage conditions and validity period:
1. Kit storage :; 2-8 ℃.
2. Validity: 6 months
Beijing Qisong Biological Technology Co., Ltd. is a professional agent for many well-known brands in the international life science field. Committed to the sales and promotion of various ELISA kits, immunohistochemical kits, primary and secondary antibodies, cytokines, biological reagents, pipettes, and consumables. Company website: Order phone
The Cargo Farm Quad(4x4 1000cc) is a small 2-person 4-wheel drive off-road vehicle, also called UTV (Utility Vehicle or Utility task vehicle), It's named because it's often used on farms. Our Farm Quad are trail ready and hard working with a dumping cargo box to facilitate the transportation of goods.
Cargo Farm Quad
Farm Quad,Farm Quad With Cargo,Cargo Farm Quad Atv,Cargo Farm Quad
Binzhou Daowang Power Co.,Ltd , https://www.dwutv.com