Incubator classification
The incubator is the main equipment for cultivating microorganisms and can be used for the cultivation and breeding of bacteria and cells. The principle is to artificially create an artificial environment for the growth and reproduction of microorganisms and cells and bacteria in the incubator, such as controlling a certain temperature, humidity, gas, and the like. The incubators currently used are mainly divided into four types: direct electrothermal incubators, water-blocking electrothermal incubators, biochemical incubators, and carbon dioxide incubators.
1. Electrothermal and water-proof incubator
1.1 Introduction to electrothermal and water-proof incubators
The outer casing of the electrothermal and water-proof incubator is usually made of asbestos board or iron-painted paint. The inner layer of the water-proof incubator is made of copper-stained water storage interlayer. The interlayer of the electro-thermal incubator is insulated with asbestos or glass wool. The material is made to enhance the heat preservation effect. The thermometer is placed on the top of the incubator and automatically controlled by the temperature controller to make the temperature inside the box constant. The water-proof incubator is heated by means of an electric heating tube, and the electrothermal incubator is directly heated by a heating wire, and the air is convected to make the temperature inside the box uniform.
On the front side of the incubator, there are indicator lights and temperature adjustment knobs. When the power is turned on, the red indicator light is on, and the rotation is rotated to the required scale according to the required temperature. After the temperature is reached, the red indicator light is extinguished. It indicates that the required temperature has been reached in the box, and the temperature inside the box is automatically controlled by the temperature controller.
1.2 Incubator use and maintenance
1. The culture in the box should not be over-squeezed in order to facilitate the convection of hot air. The items should be closed whenever the items are placed or removed to avoid temperature fluctuations.
2. The electrothermal incubator should be placed in the tank with a container of water to maintain a certain humidity.
3. The water-proof incubator should pay attention to adding water and then energizing. At the same time, the water level should be checked frequently and water should be added in time.
4. When using the electrothermal incubator, the wind top should be properly unscrewed to facilitate adjusting the temperature inside the box.
Second, biochemical incubator
1. The biochemical incubator is equipped with heating wire heating and compressor cooling. Therefore, the adaptability range is large, and the temperature can be maintained at a constant temperature throughout the year, and thus it is gradually popularized.
2. The incubator is similar to the electric heating incubator. Since the compressor is installed, also observe the precautions for maintenance of the refrigerator. For example, keep the voltage stable, do not over-tilt, and clean the dust on the radiator in time.
Third, carbon dioxide incubator
The carbon dioxide incubator is improved on the basis of ordinary culture, mainly to add CO2 to meet the environment required for cultivating microorganisms. Mainly used for tissue culture and cultivation of some special microorganisms. Installation and commissioning of CO2 incubator The front of the CO2 incubator has an operating panel with power switch, temperature regulator (manual and liquid crystal display), CO2 injection switch, CO2 adjustment knob, humidity adjustment knob, temperature Display panel, CO2 display panel and humidity display panel, carbon dioxide sample hole (for extracting the sample in the box to detect whether the CO2 in the box reaches the content displayed on the display panel) and alarm device (over temperature warning light).
1. The incubator should be placed in a relatively stable position and away from heat sources to prevent temperature fluctuations and microbial contamination.
2. Before turning on the power, add a certain amount of distilled water to the incubator according to the instruction manual (it is best to add a certain amount of disinfectant to the added water, see the instructions) to avoid burning the machine.
3. When the water is added to a certain amount, the alarm light is on, that is, the water is stopped, and the power switch is turned on, that is, the heating is started, and the temperature controller is adjusted to the required temperature.
4. When the temperature reaches the required temperature, the heating will stop automatically. When the temperature exceeds the required temperature, the over-temperature alarm light will be on and an alarm will sound.
5. The CO2 used in the incubator can use liquid CO2 or gas, no matter which kind of CO2 is used to supply the pipe, the pipe can not be too curved to ensure the smooth flow of gas. Generally use CO2 cylinders and connect the pressure control meter.
6. Zero adjustment of CO2 content After the temperature and humidity in the tank are stable (usually three days), turn the CO2 adjustment knob to adjust the number of the display panel to 0.00. After 5 minutes, repeat the adjustment until necessary. The reading on the plate is stable. Turn on the CO2 injection switch (injection light is on), adjust the CO2 setting to the desired concentration so that the concentration reaches the set concentration (injection light is off) and maintain for at least 10 minutes. Thereafter, the CO2 content in the tank is automatically adjusted by the CO2 controller.
7. CO2 incubator humidity adjustment First press the humidity adjustment knob, then turn the humidity adjustment knob to the required culture humidity, after which the humidity regulator automatically adjusts the humidity.
Four or three gas incubators
The incubator is a new product that is further improved on the basis of a carbon dioxide incubator. The principle is the same as other incubators. It is characterized by not only adding CO2, but also adding nitrogen and oxygen, and all of them are controlled and regulated by various computers. It is mainly used for the reproduction and cultivation of some special microorganisms. It is generally not widely used. At present, only a few foreign manufacturers produce it, and the price is relatively expensive.
Incubator precautions:
1. The incubator should be managed by a special person. Once any switch and adjustment knob on the operation panel is fixed, do not twist it at will, so as not to affect the temperature inside the box, the fluctuation of CO2 and humidity, and reduce the sensitivity of the machine.
2. The added water must be distilled water or non-ionized water to prevent the mineral deposits from corroding in the water tank. Water must be changed once a year. Always check if there is enough water in the box.
3. The box should be regularly cleaned and disinfected with disinfectant. The shelf can be taken out for cleaning and disinfection to prevent other microbial contamination and lead to experimental failure.
4. Regularly check the over-temperature safety device to prevent over-temperature. The method is to press the monitoring alarm button, turn the fixing screw until the over-temperature alarm device rings, and then turn off the over-temperature safety light.
5. If CO2 is not used for a long time, the CO2 switch should be turned off to prevent the CO2 regulator from malfunctioning.
6. The CO2 used must be pure, otherwise reduce the sensitivity of the CO2 sensor and contaminate the CO2 filter unit.
7. In the incubator without humidity control, in order to keep the CO2 in the tank stable, put a water container in the bottom of the box.
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