How to prepare density gradient centrifuge?

The density gradient centrifugation method is a commonly used method for separating small biological molecules. It is widely used in the separation and purification of viruses, subcellular organelles, proteins, plasmid DNA, and RNA.

Preparation of density gradient centrifugal fluid is a prerequisite for density gradient centrifugal separation.

How to prepare density gradient centrifuge? Common preparation methods include layering method, freeze-thaw method, mixing method and automatic gradient method.