Solid phase extraction device for typical SPE separation

Shanghai Xinzhuang adheres to the principle of "survive by quality, seek development by science and technology, seek efficiency by management, and seek credit by service". It has an industry-recognized team of experts and first-rate scientific talents. With a deep understanding and mastery of scientific instrument technology, On the basis of independent research and development of core technologies for scientific instruments, not only cryogenic temperature instruments, ultrasonic instruments, cabinet instruments, microbiological instruments, ice-making and refrigeration instruments, and laboratory-use supporting instruments have been developed, but also various scientific instrument technologies have been gradually formed. The product pattern of common development of reserve products. Solid-phase extraction technology of the LNB brand solid-phase extraction device developed and produced by Shanghai Xinzhuang Instrument Co., Ltd. Since the solid-phase extraction method was introduced in the late 1970s, the development of various SPE columns with different stationary phases has been rapidly developed. The solid phase extraction method is continuously adapted to the pretreatment requirements of various samples, and the solid phase extraction method for different types of compounds is gradually improved.

Solid phase extraction (Solid Phase Extraction) is the use of solid adsorbent to adsorb the target compounds in liquid samples, and the matrix and interference compounds of the sample separation, and then eluted with eluent or desorbed by heating, to achieve separation and enrichment targets The purpose of the compound. Solid-phase extraction as a sample pretreatment technology has been more and more widely used in the laboratory.

SPE is a column chromatographic separation process. There are many similarities between high performance liquid chromatography (HPLC) and the separation mechanism, stationary phase and solvent selection. However, the SPE column has a larger particle size (>40 μm) than the HPLC packing (3 to 10 μm). Due to the short column bed and large particle size, SPE column efficiency is much lower than HPLC column. A high-efficiency HPLC column can produce more than 10,000 plates, while a SPE column can obtain 10 to 50 plates. Therefore, compounds with very different properties are retained separately by SPE. In this case, the typical SPE separation is performed not in an isocratic manner but in a digitaIon-offmode. This means that with a stepped gradient, the analyte is either not adsorbed firmly by the stationary phase or is not retained at all. Another difference with HPLC is that the SPE column is for single use.

The characteristics of the SPE column itself determine its application range. The SPE technology with lower separation efficiency is mainly used to process samples. The objectives to be achieved with SPE are: (1) to remove interfering substances from the sample for subsequent analysis; (2) to enrich trace components and to increase analytical sensitivity; (3) to convert the sample solvent to analysis The methods are matched; (4) In-situ derivatization; (5) Desalting of the sample; (6) Convenient storage and transportation of the sample.

Among the above points, purification and enrichment are the most important.

More about solid-phase extraction technology consulting, please consult Shanghai Xinzhuang Instrument Co., Ltd., Shanghai Xinzhuang Instrument Co., Ltd. Senior engineer will answer for you, Shanghai Xinzhuang Instrument Co., Ltd. independent research and development of Xinzhuang (LNB) brand Solid-phase extraction devices have a wide range of application products, high stability, quality assurance, strict compliance with the credibility and commitment, is willing to win the trust of customers with the best quality and service, to create a classic quality.

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