ELISA kit is a kind of experimental diagnosis method with high sensitivity, strong specificity and good repeatability in biological tests. And its reagents are stable, easy to store, and easy to operate, so today we are going to talk about elisa kits. Let's take a look at the method steps for preparing ELISA kits.
1. Computer screening of antigen epitopes;
2. Preparation of antigen;
3. Collection of serum;
4. Establishment of indirect ELISA method;
5. Sensitivity and specificity verification of indirect ELISA method;
6. Stability verification of the kit;
7. Clinical testing of the slaughterhouse. The method is simple, fast, high sensitivity, strong specificity, qualified stability, and good repeatability. The kit prepared by the invention can effectively detect the hepatitis E virus infecting pigs, is suitable for the detection of a large number of morbidity samples, can be used for the rapid diagnosis of swine hepatitis E, and can prevent and control the epidemic and resistance of swine hepatitis E Hepatitis E virus is transmitted from pigs to humans.
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